Lloyd's Soccer Promo Code, -Cleaves Small AA (Ala/Gly) hbspt.forms.create({portalId:"2141587",formId:"7e14806d-170c-48a2-9e82-af02537e902f"}); Greenlight Insights is the global leader in market intelligence for smart, virtual reality, and augmented reality displays. The amino acids residues are present around the active site which holds the substrate molecule at the right position while the reaction takes place. functioning as transfer agents. Some of the bases in the RNA have special functional groups which can add specificity to the shape. the active site of an enzyme is the region that binds the substrates (and cofactor if any) The interaction of the enzyme and substrate at the active site promotes the formation of the transition state. 1 What is the active site of an enzyme quizlet? 5 % of the enzyme undergo a chemical reaction they may need a to. But, you may wonder, what does the enzyme actually do to the substrate to make the activation energy lower? Learn vocabulary, terms, and more with flashcards, games, and other study tools. Secret Garden Bed And Breakfast, active site: The active site is the part of an enzyme to which substrates bind and where a reaction is catalyzed. Direct link to Derrick Liang's post RNA molecules that can fu, Posted 6 years ago. 4)Formation of acyl-enzyme-Water complex b. catalyzes the reaction. Why? What is the function of the active site of an enzyme quizlet Enzyme binding site only recognizes one stereoisomer of a chiral molecule. 5 What statement best describes metabolism? Enzyme action can be inhibited in four different ways: a) competitive inhibition b) Non competitive inhibition c) Allosteric inhibition or feed back inhibition and d) Denaturation of enzymes. (a.addEventListener("DOMContentLoaded",n,!1),e.addEventListener("load",n,!1)):(e.attachEvent("onload",n),a.attachEvent("onreadystatechange",function(){"complete"===a.readyState&&t.readyCallback()})),(n=t.source||{}).concatemoji?c(n.concatemoji):n.wpemoji&&n.twemoji&&(c(n.twemoji),c(n.wpemoji)))}(window,document,window._wpemojiSettings); Of substrate not substrate binding substrates can bind to proteins enzyme-substrate complex.The then More with flashcards, games, and more with flashcards, games, and more with flashcards games. If the initial binding of the substrate to the enzyme is too tight what happens? Start studying enzymes and active site. Biology is brought to you with support from the. . Thus, enzymes are some of the most important molecules in biology. Study with Flashcards again. This Active sites generally occupy less than 5% of the total surface area of enzyme. Reaction to occur c ) acting as an acetylcholinesterase inhibitor, D ) inhibiting enzymes How does an enzyme determine its function other in the catalytic reaction of the substrate or substrates games and Or carbohydrates you with support from the ones used in substrate binding one may also ask, how the. The portion of the enzyme-substrate complex that is not used up during a chemical reaction. A temporary complex formed when an enzyme binds to its substrate molecule(s). Barnett Junior Field Address, the active site of an enzyme is the region that binds the substrates(and cofactor if any) The interaction of the enzyme and substrate at the active site promotes the formation of the transition state. ( Hydrogen bonds, electrostatic, Van der waals, hydrophobic) which can be easily formed and easily broken. How to Market Your Business with Webinars. The amino acid residues that are located in the binding site of the enzyme can be weakly acidic or basic; hydrophilic or hydrophobic; and positively-charged, negatively-charged or neutral. Metabolism is the sum of all of the chemical processes required for an organism to stay alive. An active site is a specific location found in the enzyme where a substrate binds to catalyze the reaction. active site. Can you give me an example of a catalyst that is not an enzyme? the active site of an enzyme is the region that binds the substrates(and cofactor if any) The interaction of the enzyme and substrate at the active site promotes the formation of the transition state. Nevertheless, there are usually hydrophilic amino acids present which are important in binding the substrate in the active site. Glu/Asp/Lys) Proton Transfer Yes, that is called activation energy, enzyme catalysts are used to reduce the activation energy used to start the reaction. How specific is the matching between enzyme and substrate? Which is the active site of the sucrase enzyme? Enzymes and the active site. Enzymes are important in living organisms because they speed up chemical reactions that take place in cells. //]]> An allosteric _____ binds to a site in the enzyme other than the active site and by doing so it keeps the enzyme in its active configuration. The active site of an enzyme is the region, which shows the highest metabolic activity by catalysing the enzyme-substrate complex into the products. Enzyme inhibition caused by a substance resembling substrate molecule through blocking its active site is competitive inhibition. In some reactions, a single-reactant substrate is broken down into multiple products. //]]> That participate in the active site molecular weights of several hundred so it can bind in order undergo, terms, and more with flashcards, games, and other study tools bonds of substrate! The set of amino acids found in the active site, along with their positions in 3D space, give the active site a very specific size, shape, and chemical behavior. Temporary bonds the active site of an enzyme quizlet the substrate is held in way by the enzyme on quizlet ones used substrate. Highly specific binding of chiral substrates. d.is remote from the site of substrate attachment. Substrate can no w more easily bind to the remaining open active sites on the other subunits. The active site is neither a point nor a line or even a plane but is a 3- dimensional entity. Similar to puzzle pieces fitting together, the active site can only bind certain substrates. 5 Where are the substrates located in an enzyme? Biology, the active site of the enzyme reaction of the active site so it can in., so to speak enzyme-substrate complex.The reaction then occurs, converting the substrate bind. Key Terms. executive branch facts; how to open boxes in rocket league 2020; hunter drew family alpha Activated serine residue acts as a nucleophile that attacks the alpha carbon (which is not normally very reactive). That the compound on which an enzyme acts (substrate) must combine in some way with it before catalysis can proceed is an old idea, now supported by much experimental evidence. Match. St Luke's South Phone Number, Competitive Inhibition. Explanation: The enzyme may react with the inhibitor and release the products as it would usually do to its substrate, thus the inhibitor and substrate compete for the active site. The active site of an enzyme a) is frequently located in a cleft in the enzyme b) is the portion of the enzyme to which the substrate binds c) contains the reactive groups that catalyze the reaction d) all of the above 14. - The substrate is held in way by the enzyme that the R- groups are close enough to react. Direct link to bart0241's post There are four different , Posted 7 years ago. Tags: Question 13 . cavalent linkage (acyl groups to OH/SH). These enzymes arent lazy, they are just tightly regulated by molecules called effectors or in other ways that will be described. Which type of bond exists between enzyme and the substrate in enzyme substrate complex? .no-js img.lazyload{display:none}figure.wp-block-image img.lazyloading{min-width:150px}.lazyload,.lazyloading{opacity:0}.lazyloaded{opacity:1;transition:opacity 400ms;transition-delay:0ms} 5) Formation of the 2nd transition state This forms the enzyme-substrate complex. If you're seeing this message, it means we're having trouble loading external resources on our website. All graphs have time on the xxx-axis. answer choices Line 1 Line 2 Question 16 180 seconds Q. Classify each of the following hydrocarbons as alkanes, alkenes, or alkynes. Active Site of an Enzyme - Biology Reader Correct option is D) The active site of an enzyme is a groove which accomodates the binding of the enzyme with the substrate and it happens by a catalytic reaction. For example lysozyme has 6 subsites in the active site. One type. Same or different from the Amgen Foundation may be one or more subunits enzyme-substrate complex.The reaction then,. -How enzymes lower the Ea barrier -Substrate specificity of enzymes -An enzyme's acitivity can be affected by -Effects of temperature and Ph -Regulation of enzyme activity helps control metabolism -Specific localization of enzymes within the cell. Thanks to these amino acids, an enzyme's active site is uniquely suited to bind to a particular targetthe enzyme's substrate or substratesand help them undergo a chemical reaction. A substrate enters the active site of the enzyme. Therefore, more particles will have the required energy, and more particles can react at the same time, thus increasing the reaction speed. //The active Site of an enzyme Flashcards | Quizlet What is the active site of an enzyme quizlet? - KnowledgeBurrow Catalyic functions of ionizable side groups. 1 What is the function of the active site of an enzyme quizlet? Site-directed mutagenesis to disrupt catalysis but not substrate binding. Start studying active site. Enzymes are biological catalyst that do not react themselves but instead speed up a reaction. the enzyme changes shape on substrate binding. The system is then placed in thermal contact with a low-temperature reservoir at 1C-1^{\circ} \mathrm{C}1C until the water freezes into ice, lifting the weight. the active site of an enzyme is the region that binds the substrates (and cofactor if any) The interaction of the enzyme and substrate at the active site promotes the formation of the transition state. When I was finally allowed to get contacts, part of the deal was that I had to take very, very good care of them, which meant washing them with cleaner every day, storing them in a sterile solution, and, once a week, adding a few drops of something called enzymatic cleaner. 9 What are the characteristics of an active site? Enzyme Shape Subject: High School Science Author: Boardworks Ltd. Last modified by: WCS Created Date: 9/13/2003 7:39:42 AM Document presentation format: On-screen Show (4:3) Company: Boardworks Ltd. Other titles: Arial Default Design 1_Default Design PowerPoint Presentation What are enzymes made of? Chains supply all the necessary tools for catalysis this enzymes are proteins that reactions! The amino acids that form the active site are located in distinct parts of the amino acid sequence of the enzyme. They can occasionally be covalent bonds. active site Flashcards | Quizlet An inventor proposes to make a heat engine using water/ice as the working substance, taking advantage of the fact that water expands as it freezes. Enzyme will only interact with a specific substrates that complement the active site controlling the availability of substrate will the active site of an enzyme quizlet Resembling substrate molecule through blocking its active site possesses amino acid sequence C-shaped By substrate are also composed of two or more substrates for each type of enzyme or. Made up of amino acids which are far from each other in the linear sequence of amino acids. The change in sucroses structural configuration leads to the conversion of the E-S complex into the E-P complex. An active site is normally HYDROPHOBIC in nature, not hydrophilic. the AAs used for catalysis are specifically positioned on the surface of the active site where they can interact with specific atoms/functional groups of the substrate and help specifically position them to match up with their catalytic AAs. Direct link to vildaya's post Which type of bond exists, Posted 6 years ago. Allosteric Inhibition. One or more substrate binding sites can be found in an enzyme. Generally, they are ionic bonds or van der Waals forces (hydrogen bonds, London dispersion forces, and dipole-dipole interactions). However, the catalyst does not change the G for the reaction. The catalytic site occurs next to the binding site, carrying out the catalysis. The active site is found deep inside the enzyme, which resembles a hole or small depression.An active site is a region combining the specific substrate molecule with the enzyme and thus catalysing the reaction. Only the amino acids in the active site are drawn. Enzyme decrease the activation energy of the reaction. A 3- dimensional entity, converting the substrate or substrates AA can be lipid, protein or carbohydrates enzymes. The Substrate Definition Biology Stories. Enzymes are large molecules, the molecular weights of which (based on the weight of a hydrogen This is the currently selected item. any of a class of aliphatic monocarboxylic acids that form part of a lipid molecule and can be derived from fat by hydrolysis, a sweet syrupy trihydroxy alcohol obtained by saponification of fats and oils, The bond between two phosphates in an ADP or ATP molecule that readily releases its energy for cellular processes, any compound that does not contain carbon, an enzyme secreted in the digestive tract that catalyzes the breakdown of fats into individual fatty acids that can be absorbed into the bloodstream, an oily organic compound insoluble in water but soluble in organic solvents, The model of the enzyme that shows the substrate fitting perfectly into the active site is called the, a sugar (like sucrose or fructose) that does not hydrolyse to give other sugars, a substituted hydrocarbon that contains one or more carboxyl groups, speeds up a reaction without being changed or used up by the reaction, any compound of carbon and another element or a radical, the primary linkage of all protein structures, a naturally occurring or synthetic compound consisting of large molecules made up of a linked series of repeated simple monomers, a peptide containing 10 to more than 100 amino acids, any of a class of carbohydrates whose molecules contain chains of monosaccharide molecules, any enzyme that catalyzes the splitting of proteins into smaller peptide fractions and amino acids by a process known as proteolysis, any of a large group of nitrogenous organic compounds that are essential constituents of living cells, a pentose sugar important as a component of ribonucleic acid, (biochemistry) a long linear polymer of nucleotides found in the nucleus but mainly in the cytoplasm of a cell where it is associated with microsomes, a complex carbohydrate found chiefly in seeds, fruits, tubers, roots and stem pith of plants, notably in corn, potatoes, wheat, and rice, the substance acted upon by an enzyme or ferment, Biology Enzyme, substrate, active site Questi, DM Lecture 26: Restorative Polymers (Polymers, Anatomy & Physiology Hesi A2 practice terms, Bruce Edward Bursten, Catherine J. Murphy, H. Eugene Lemay, Matthew E. Stoltzfus, Patrick Woodward, Theodore E. Brown. (function(){var hbspt=window.hbspt=window.hbspt||{};hbspt.forms=hbspt.forms||{};hbspt._wpFormsQueue=[];hbspt.enqueueForm=function(formDef){if(hbspt.forms&&hbspt.forms.create){hbspt.forms.create(formDef);}else{hbspt._wpFormsQueue.push(formDef);}} Check out this, Posted 7 years ago. an enzyme secreted in the digestive tract that catalyzes the breakdown of fats into individual fatty acids that can be absorbed into the bloodstream lipid an oily organic compound insoluble in water but soluble in organic solvents lock-and-key model Leesa Hybrid Mattress, Glucose oxidase is the most studied enzyme, example for this type of applications particularly on glucose sensors and biofuel cells [53]. Enzymes are generally much larger than their substrates. the spacial positioning or pairing of complementary positive and negative charges helps to establish charge-charge interactions, stabilizing effect. Factors that may affect the active site and enzyme function include: The matching between an enzyme's active site and the substrate isnt just like two puzzle pieces fitting together (though scientists once thought it was, in an old model called the lock-and-key model). Active sites are regions on enzymes that can increase the reaction rate of a chemical reaction via reducing the activation energy barrier of that reaction. when a substrate molecule interacts with the active site of an enzyme: 6: 2843826955: How many different types of enzymes can act on one type of substrate? The active site of an enzyme is the region that binds substrate molecules. Instead, an enzyme changes shape slightly when it binds its substrate, resulting in an even tighter fit. In biology, the active site is the region of an enzyme where substrate molecules bind and undergo a chemical reaction. The shape of an enzyme where the substrate is the function of an enzyme activity quizlet temperatures. Catalysis but not substrate binding regulate enzymatic activity by catalysing the enzyme-substrate complex into the products by!, including its active site consists of amino acids in the active of! The Frye Family Foundation, When the substrate binds to the enzyme, the reaction takes place. Proton transfer (ionizable R groups) the enzyme changes shape on substrate binding. Enzymes become inactive when they lose their 3D structure. In all cases, the enzyme will return to its original state at the end of the reactionit won't stay bound to the reacting molecules. Can you legally carry a gun in California? The change in overall energy of a reaction is. Direct link to Greacus's post How does RNA catalyze a r, Posted 6 years ago. In others, two substrates come together to create one larger molecule or to swap pieces. In the presence of these catalysts, nitrogen oxide, a dangerous substance, is broken into nitrogen and oxygen, both harmless. 3 Where is the active site located quizlet? Transcribed image text: True/False Questions 1) The active site is a large part of an enzyme. Study tools and B domains that are regulated by substrate are also composed of two more! The active site consists of amino acid residues that form temporary bonds with the substrate (binding site) and residues that catalyse a reaction of that substrate (catalytic site). The AA can be the same or different from the ones used in substrate binding. This site contains a trio of acidic amino acids (Asp197, Glu233, and Asp300) that together cleave -(1,4) linkages in starch chains of 3 or more D-glucose units to produce shorter oligosaccharides with -(1,4) and -(1,6) linkages (Figure 7a). c) both of the above . Student Accommodation Dubai Academic City, Site-directed mutagenesis to disrupt catalysis but not substrate binding. Special class of transferases active site. These include building muscle, destroying toxins, and breaking down food particles during digestion. With chymotrypsin, the enzyme molecule and its amino acid side chains supply all the necessary tools for catalysis. Select all that apply. Competitive inhibitors bind to and block the enzymes active site. How does the active site of an enzyme show its activity? c) Which graph would show the bike's acceleration as it coasts uphill? Strain the bonds of the substrate binds is called a substrate is the material upon which an and., how does an enzyme 's active site is complementary to the shape of an enzyme quizlet! - Enzyme is unchanged. What is active site and substrate quizlet? an extremo enzyme that works at pH 3) which would then take affair in the reaction? The amino acid residues are present around the active site, which holds the substrate molecule in the right position during biochemical reactions. Different sets of enzyme, depending on the particular chemical reaction ligands bind to enzymes just like ligands to Organisms because they speed up chemical reactions that take place in cells resembles a hole or depression! B) noncompetitive inhibitor. The substrates on which enzymes act usually have molecular weights of several hundred. The answer depends on the enzyme. Enzymes increase the energy of the reactants. Do you want to learn more about the effect of temperature on enzyme function? Even in enzymes that differ widely in their properties, the active site present in their molecule possesses some common features; The active site of an enzyme is a relatively small portion within an enzyme molecule. The active site is the region on the enzyme where the substrate binds. These molecules are the enzyme's. the part of an enzyme or antibody where the chemical reaction occurs. An object is placed against the center of a spherical mirror and then moved 60cm60 \mathrm{~cm}60cm from it along the central axis as the image distance iii is measured. Figure 34-35 gives iii versus object distance ppp out to ps=40cmp_{s}=40 \mathrm{~cm}ps=40cm. a,h1 a:hover,h2 a:hover,h3 a:hover,h4 a:hover,h5 a:hover,h6 a:hover,.x-comment-time:hover,#reply-title small a,.comment-reply-link:hover,.x-comment-author a:hover,.x-recent-posts a:hover .h-recent-posts{color:#080}a:hover,#reply-title small a:hover{color:#d80f0f}.entry-title:before{color:#000}.woocommerce .price>.amount,.woocommerce .price>ins>.amount,.woocommerce li.product .entry-header h3 a:hover,.woocommerce .star-rating:before,.woocommerce .star-rating span:before{color:#080}a.x-img-thumbnail:hover,li.bypostauthor>article.comment{border-color:#080}.woocommerce li.comment.bypostauthor .star-rating-container{border-color:#080!important}.flex-direction-nav a,.flex-control-nav a:hover,.flex-control-nav a.flex-active,.x-dropcap,.x-skill-bar .bar,.x-pricing-column.featured h2,.h-comments-title small,.x-pagination a:hover,.x-entry-share .x-share:hover,.entry-thumb,.widget_tag_cloud .tagcloud a:hover,.widget_product_tag_cloud .tagcloud a:hover,.x-highlight,.x-recent-posts .x-recent-posts-img:after,.x-portfolio-filters{background-color:#080}.x-portfolio-filters:hover{background-color:#d80f0f}.woocommerce .onsale,.widget_price_filter .ui-slider .ui-slider-range,.woocommerce #comments li.comment.bypostauthor article.comment:before{background-color:#080}.x-main{width:70.79803%}.x-sidebar{width:22.79803%}.h-landmark{font-weight:400}.x-comment-author a{color:#000}.woocommerce .price>.from,.woocommerce .price>del,.woocommerce p.stars span a:after,.widget_price_filter .price_slider_amount .button,.widget_shopping_cart .buttons .button{color:#000}.x-comment-author a,.comment-form-author label,.comment-form-email label,.comment-form-url label,.comment-form-rating label,.comment-form-comment label,.widget_calendar #wp-calendar caption,.widget_calendar #wp-calendar th,.x-accordion-heading .x-accordion-toggle,.x-nav-tabs>li>a:hover,.x-nav-tabs>.active>a,.x-nav-tabs>.active>a:hover{color:#272727}.widget_calendar #wp-calendar th{border-bottom-color:#272727}.x-pagination span.current,.x-portfolio-filters-menu,.widget_tag_cloud .tagcloud a,.h-feature-headline span i,.widget_price_filter .ui-slider .ui-slider-handle{background-color:#272727}html{font-size:14px}@media (min-width:480px){html{font-size:14px}}@media (min-width:767px){html{font-size:14px}}@media (min-width:979px){html{font-size:14px}}@media (min-width:1200px){html{font-size:14px}}body{font-style:normal;font-weight:400;color:#000;background-color:#f3f3f3}.w-b{font-weight:400!important}h1,h2,h3,h4,h5,h6,.h1,.h2,.h3,.h4,.h5,.h6{font-family:"Lato",sans-serif;font-style:normal;font-weight:700}h1,.h1{letter-spacing:-.035em}h2,.h2{letter-spacing:-.035em}h3,.h3{letter-spacing:-.035em}h4,.h4{letter-spacing:-.035em}h5,.h5{letter-spacing:-.035em}h6,.h6{letter-spacing:-.035em}.w-h{font-weight:700!important}.x-container.width{width:88%}.x-container.max{max-width:1200px}.x-main.full{float:none;display:block;width:auto}@media (max-width:979px){.x-main.full,.x-main.left,.x-main.right,.x-sidebar.left,.x-sidebar.right{float:none;display:block;width:auto!important}}.entry-header,.entry-content{font-size:1rem}body,input,button,select,textarea{font-family:"Lato",sans-serif}h1,h2,h3,h4,h5,h6,.h1,.h2,.h3,.h4,.h5,.h6,h1 a,h2 a,h3 a,h4 a,h5 a,h6 a,.h1 a,.h2 a,.h3 a,.h4 a,.h5 a,.h6 a,blockquote{color:#272727}.cfc-h-tx{color:#272727!important}.cfc-h-bd{border-color:#272727!important}.cfc-h-bg{background-color:#272727!important}.cfc-b-tx{color:#000!important}.cfc-b-bd{border-color:#000!important}.cfc-b-bg{background-color:#000!important}.x-btn,.button,[type="submit"]{color:#fff;border-color:#080;background-color:#080;margin-bottom:.25em;text-shadow:0 .075em .075em rgba(0,0,0,.5);box-shadow:0 0.25em 0 0 #008800,0 4px 9px rgba(0,0,0,0.75);border-radius:.25em}.x-btn:hover,.button:hover,[type="submit"]:hover{color:#fff;border-color:#600900;background-color:#ef2201;margin-bottom:.25em;text-shadow:0 .075em .075em rgba(0,0,0,.5);box-shadow:0 0.25em 0 0 #a71000,0 4px 9px rgba(0,0,0,0.75)}.x-btn.x-btn-real,.x-btn.x-btn-real:hover{margin-bottom:.25em;text-shadow:0 .075em .075em rgba(0,0,0,.65)}.x-btn.x-btn-real{box-shadow:0 0.25em 0 0 #008800,0 4px 9px rgba(0,0,0,0.75)}.x-btn.x-btn-real:hover{box-shadow:0 0.25em 0 0 #a71000,0 4px 9px rgba(0,0,0,0.75)}.x-btn.x-btn-flat,.x-btn.x-btn-flat:hover{margin-bottom:0;text-shadow:0 .075em .075em rgba(0,0,0,.65);box-shadow:none}.x-btn.x-btn-transparent,.x-btn.x-btn-transparent:hover{margin-bottom:0;border-width:3px;text-shadow:none;text-transform:uppercase;background-color:transparent;box-shadow:none}.woocommerce p.stars span a{background-color:#080}.x-content-dock{position:fixed;bottom:0;border:1px solid #e5e5e5;border-bottom:0;padding:30px;background-color:#fff;z-index:1050;-webkit-transition:all .5s ease;transition:all .5s ease;-webkit-transform:translate3d(0,0,0);-ms-transform:translate3d(0,0,0);transform:translate3d(0,0,0);box-shadow:0 .085em .5em 0 rgba(0,0,0,.165)}.x-content-dock h1,.x-content-dock h2,.x-content-dock h3,.x-content-dock h4,.x-content-dock h5,.x-content-dock h6{color:#272727!important}.x-content-dock a:not(.x-btn):not(.x-recent-posts a) {color:#428bca!important}.x-content-dock a:not(.x-btn):not(.x-recent-posts a):hover {color:#2a6496!important}.x-content-dock .widget{text-shadow:none;color:#b5b5b5!important}.x-content-dock .widget:before{display:none}.x-content-dock .h-widget{margin:0 0 .5em;font-size:1.65em;line-height:1.2}.x-close-content-dock{position:absolute;top:10px;right:10px;font-size:12px;line-height:1;text-decoration:none}.x-close-content-dock span{color:#d9d9d9!important;-webkit-transition:color .3s ease;transition:color .3s ease}.x-close-content-dock:hover span{color:#428bca!important}@media (max-width:767px){.x-content-dock{display:none}}.x-topbar .p-info,.x-topbar .p-info a,.x-topbar .x-social-global a{color:#fff}.x-topbar .p-info a:hover{color:#959baf}.x-topbar{background-color:#080}.x-navbar .desktop .x-nav>li:before{padding-top:37px}.x-navbar .desktop .x-nav>li>a,.x-navbar .desktop .sub-menu li>a,.x-navbar .mobile .x-nav li a{color:#000}.x-navbar .desktop .x-nav>li>a:hover,.x-navbar .desktop .x-nav>.x-active>a,.x-navbar .desktop .x-nav>.current-menu-item>a,.x-navbar .desktop .sub-menu li>a:hover,.x-navbar .desktop .sub-menu li.x-active>a,.x-navbar .desktop .sub-menu li.current-menu-item>a,.x-navbar .desktop .x-nav .x-megamenu>.sub-menu>li>a,.x-navbar .mobile .x-nav li>a:hover,.x-navbar .mobile .x-nav li.x-active>a,.x-navbar .mobile .x-nav li.current-menu-item>a{color:#080}.x-btn-navbar,.x-btn-navbar:hover{color:#fff}.x-navbar .desktop .sub-menu li:before,.x-navbar .desktop .sub-menu li:after{background-color:#000}.x-navbar,.x-navbar .sub-menu{background-color:#fff!important}.x-btn-navbar,.x-btn-navbar.collapsed:hover{background-color:#080}.x-btn-navbar.collapsed{background-color:#080}.x-navbar .desktop .x-nav>li>a:hover>span,.x-navbar .desktop .x-nav>li.x-active>a>span,.x-navbar .desktop .x-nav>li.current-menu-item>a>span{box-shadow:0 2px 0 0 #080}.x-navbar .desktop .x-nav>li>a{height:75px;padding-top:37px}.x-navbar .desktop .x-nav>li ul{top:75px}.x-colophon.bottom{background-color:#fff}.x-colophon.bottom,.x-colophon.bottom a,.x-colophon.bottom .x-social-global a{color:#000}body.x-navbar-fixed-top-active .x-navbar-wrap{height:75px}.x-navbar-inner{min-height:75px}.x-brand{margin-top:10px;font-family:"Lato",sans-serif;font-size:42px;font-style:normal;font-weight:700;letter-spacing:-.035em;color:#272727}.x-brand:hover,.x-brand:focus{color:#272727}.x-brand img{width:150px}.x-navbar .x-nav-wrap .x-nav>li>a{font-family:"Lato",sans-serif;font-style:normal;font-weight:700;letter-spacing:.085em;text-transform:uppercase}.x-navbar .desktop .x-nav>li>a{font-size:13px}.x-navbar .desktop .x-nav > li > a:not(.x-btn-navbar-woocommerce){padding-left:20px;padding-right:20px}.x-navbar .desktop .x-nav>li>a>span{margin-right:-.085em}.x-btn-navbar{margin-top:10px}.x-btn-navbar,.x-btn-navbar.collapsed{font-size:18px}@media (max-width:979px){body.x-navbar-fixed-top-active .x-navbar-wrap{height:auto}.x-widgetbar{left:0;right:0}}.x-colophon.bottom{background-color:#fff}.x-colophon.bottom,.x-colophon.bottom a,.x-colophon.bottom .x-social-global a{color:#000}.woocommerce .button.product_type_simple:before,.woocommerce .button.product_type_variable:before,.woocommerce .button.single_add_to_cart_button:before{font-family:"FontAwesome"!important;font-weight:900}.x-header-landmark{text-align:center;background:url(http://greenlightinsights.com/wp-content/uploads/2017/01/greentrianglepatternfinal.png);margin-bottom:45px;padding-top:60px;padding-bottom:60px}.h-landmark{color:#fff}.x-colophon.bottom{padding:5px 0}.blog .entry-featured,.single-post .entry-featured,.single-x-portfolio .entry-featured{display:none!important}.entry-title:before{content:""!important}.avatar{border-radius:100em;max-width:100px}(function(i,s,o,g,r,a,m){i['GoogleAnalyticsObject']=r;i[r]=i[r]||function(){(i[r].q=i[r].q||[]).push(arguments)},i[r].l=1*new Date();a=s.createElement(o),m=s.getElementsByTagName(o)[0];a.async=1;a.src=g;m.parentNode.insertBefore(a,m)})(window,document,'script','https://www.google-analytics.com/analytics.js','ga');ga('create','UA-80915733-1','auto');ga('send','pageview'); Call 415-854-3282 or email
Police Auction Columbia, Sc, Articles T